Journal: Immunity

Article Title: Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans

doi: 10.1016/j.immuni.2020.07.003

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD303 147Sm, clone 201A , Fluidigm , Cat# 3147009B.

Techniques: Purification, Recombinant, Concentration Assay, Saline, Staining, Antibody Labeling, RNA Sequencing, Software, Sterility

Journal: Nature Communications

Article Title: TNF blockade induces a dysregulated type I interferon response without autoimmunity in paradoxical psoriasis

doi: 10.1038/s41467-017-02466-4

Figure Lengend Snippet: Plasmacytoid dendritic cell (pDC)-derived type I interferon controlled by TNF. a Representative immunohistochemical CD123 (IL3RA)-staining of skin from a patient with paradoxical psoriasis. b Representative confocal laser scanning microscopy of paradoxical psoriasis stained for BDCA2 (CLEC4C) (green), CD123 (red), and DAPI (blue) shows pDCs co-staining for BDCA2 and CD123 (yellow) and CD123 single-positive endothelial cells (*, red). c Histological quantification of CD123-positive pDCs per total dermal infiltrate in skin from healthy donors, psoriasis, and paradoxical psoriasis. d Correlation of numbers of CD123-positive pDCs with gene expression of IFNA2 . e IFN-α produced by pDCs enriched from peripheral blood mononuclear cells of healthy volunteers 48 h after stimulation with DNA/LL37 complexes with or without addition of anti-TNF antibodies. f Relative amount of IFN-α produced by pDCs from healthy volunteers at 48 h, unstimulated or upon stimulation with DNA-LL37 complexes with or without anti-TNF antibodies, with or without addition of TNF. g Relative IFNA2 and TNF mRNA expression by pDCs isolated from healthy volunteers, stimulated with DNA/LL37, and kept in culture for 6, 24, 48, or 72 h, respectively. f Relative IFNA2 mRNA expression by pDCs from healthy volunteers 6, 24, 48, and 72 h upon stimulation with DNA/LL37 complexes either with anti-TNF antibodies or addition of TNF. Dots represent individual patient/healthy donor ( c , d ) and horizontal bar denotes the mean value ( c ). Data in f depicted as relative expression (percentage) over amount of IFN-α produced upon stimulation with LL37/DNA (set at 100%); data shown as mean ± SD of six independent experiments with blood from six healthy volunteers (for DNA/LL37 + anti-TNF + TNF; n = 3). Data in g and h depicts one representative of four independent experiments with cells from four different healthy individuals. Statistical analysis was performed in c with unpaired Student’s t -test and in e and f with paired Student’s t -test, in d the Spearman’s rank-correlation coefficient was calculated

Article Snippet: Frozen skin sections were fixed with 4% paraformaldehyde and stained for BDCA2 (anti-BDCA2-biotin, clone AC144, Miltenyi, plus streptavidin-Alexa488) and for CD123 (anti-CD123–phycoerythrin, clone 7G3, BD Biosciences).

Techniques: Derivative Assay, Immunohistochemical staining, Staining, Confocal Laser Scanning Microscopy, Gene Expression, Produced, Expressing, Isolation

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Comparative immunoprofiling of polymyositis and dermatomyositis muscles

doi:

Figure Lengend Snippet: Antibodies employed in the present study

Article Snippet: Finally, the sections were sealed in neutral balsam. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Dilution Company MHC-I Mouse anti-human monoclonal 1:100 BioLegend MHC-II Rabbit anti-human monoclonal 1:100 Abcam CD4 Mouse anti-human monoclonal 1:100 BD Biosciences CD8 Mouse anti-human monoclonal 1:200 BD Biosciences CD303 Mouse anti-human monoclonal 1:100 BD Biosciences MAC Mouse anti-human monoclonal 1:100 Abcam Open in a separate window Antibodies employed in the present study

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Comparative immunoprofiling of polymyositis and dermatomyositis muscles

doi:

Figure Lengend Snippet: Frequencies of inflammatory cells in IIMs and NIMs

Article Snippet: Finally, the sections were sealed in neutral balsam. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Dilution Company MHC-I Mouse anti-human monoclonal 1:100 BioLegend MHC-II Rabbit anti-human monoclonal 1:100 Abcam CD4 Mouse anti-human monoclonal 1:100 BD Biosciences CD8 Mouse anti-human monoclonal 1:200 BD Biosciences CD303 Mouse anti-human monoclonal 1:100 BD Biosciences MAC Mouse anti-human monoclonal 1:100 Abcam Open in a separate window Antibodies employed in the present study

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Comparative immunoprofiling of polymyositis and dermatomyositis muscles

doi:

Figure Lengend Snippet: Proportions of inflammatory cells in IIMs and NIMs. CD4+, CD8+ and CD303+ cells were more frequent in IIMs than in NIMs (A). In PM (B), the majority of inflammatory cells were CD8+ cells. In DM (C) and NIMs (D), inflammatory cells were mainly CD4+ cells. The mean and SEM of the samples are indicated. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Finally, the sections were sealed in neutral balsam. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Dilution Company MHC-I Mouse anti-human monoclonal 1:100 BioLegend MHC-II Rabbit anti-human monoclonal 1:100 Abcam CD4 Mouse anti-human monoclonal 1:100 BD Biosciences CD8 Mouse anti-human monoclonal 1:200 BD Biosciences CD303 Mouse anti-human monoclonal 1:100 BD Biosciences MAC Mouse anti-human monoclonal 1:100 Abcam Open in a separate window Antibodies employed in the present study

Techniques:

Journal: Immunity

Article Title: Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans

doi: 10.1016/j.immuni.2020.07.003

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD303 147Sm, clone 201A , Fluidigm , Cat# 3147009B.

Techniques: Purification, Recombinant, Concentration Assay, Saline, Staining, Antibody Labeling, RNA Sequencing, Software, Sterility